Separation of di- and trisaccharide mixtures by comprehensive two-dimensional liquid chromatography. Application to prebiotic oligosaccharides
نویسندگان
چکیده
منابع مشابه
Comprehensive two-dimensional liquid chromatography for the separation of protonated and deuterated polystyrene.
Liquid chromatography at critical conditions (LCCC) has been shown to be a powerful method for the separation of complex polymers regarding chemical composition, functionality, or molecular topology. LCCC has never been used, however, to separate polymers according to the degree of deuteration. This is a very challenging task since polymers shall be separated that are identical regarding molar ...
متن کاملSeparation and identification of compounds in Rhizoma chuanxiong by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry.
A comprehensive two-dimensional liquid chromatographic separation system based on the combination of a CN column and an ODS column is developed for the separation of components in a traditional Chinese medicine (TCM) Rhizoma chuanxiong. Two columns are coupled by a two-position, eight-port valve equipped with two storage loops and controlled by a computer. The effluent is detected by both the d...
متن کاملOrthogonality of separation in two-dimensional liquid chromatography.
Two-dimensional liquid chromatography is often used to reduce the proteomic sample complexity prior to tandem mass spectrometry analysis. The 2D-LC performance depends on the peak capacity in both chromatographic dimensions, and separation orthogonality. The peak capacity and selectivity of many LC modes for peptides is not well known, and mathematical characterization for orthogonality is unde...
متن کاملSeparation of disaccharides by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry. Application to honey analysis.
A new method based on comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC×GC-ToF MS) has been developed for the first time for the analysis of complex mixtures of disaccharides previously converted to their trimethylsilyl oximes (TMSO). Among the different experimental parameters considered for optimization, both the column set combination and the di...
متن کاملCorrespondence Quantification in Comprehensive Two-Dimensional Liquid Chromatography
This correspondence corrects the description in a recent paper by Mondello et al., “Quantification in Comprehensive Two-Dimensional Liquid Chromatography” [Mondello, L.; Herrero, M.; Kumm, T.; Dugo, P.; Cortes, H.; Dugo, G. Anal. Chem., 2008, 80, 5418-5424], of previous research on peak integration. This correspondence also shows that the peak integration method proposed in that paper is equiva...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytica Chimica Acta
سال: 2019
ISSN: 0003-2670
DOI: 10.1016/j.aca.2019.01.040